Fig 5. KPn infection causes cell death.

Peritoneal neutrophils uninfected or infected with KPn (MOI 10). At indicated times, dead cells were enumerated by staining with LIVE/DEADFixable Near-IR Dead Cell Stain Kit (Invitrogen) per manufacturer’s instructions. Cells were then washed and analyzed by flow cytometry. Data in (A) is mean ± SEM from 2 independent experiments with 3–5 replicates each time. Representative dot plots are shown in (A’). Student’s t test was used for statistical analysis (p*<0.05). ns, not significant.