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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Leukoc Biol. 2018 Feb 2;103(5):909–918. doi: 10.1002/JLB.3AB0616-251RRR

Figure 3: Changes in immune function, but not recruitment, lead to improved bacterial clearance in the lung following burn injury.

Figure 3:

Mice were infected intratracheally with 1× 106 CFU of PAK at 14 dpi and samples were collected 48 h later. A) Bacterial burden in whole lung homogenate was observed in burn mice compared with sham mice. B) DHR MFI of gated pulmonary neutrophils or macrophages from both stimulated and unstimulated samples was measured by flow cytometry. Unstimulated values are shown, as well as the stimulation index (DHR MFI stimulated / DHR MFI unstimulated) C) MFI of PE-labeled TLR4 on gated neutrophils and TLR2 on gated macrophages was determined by flow cytometry in samples from lung. Representative flow cytometry plots used to derive MFI are shown under each graph of untreated burn and sham injured mice. Data shown are +/−SEM. *p<0.05, **p<0.01 and representative of three repeated experiments. Numbers of mice in representative figures; A) sham, n=4; burn, n=6; B) sham, n=4; burn, n=5; C) sham, n=5; burn, n=6.