Mice underwent an intra-tracheal inoculation with 1 × 106 CFU PAK at 14 dpi., with or without Rapamycin treatment. A) MFI of PE-labeled TLR2 on neutrophils and macrophages from whole lung cell suspensions was determined by flow cytometry. B-C), DHR MFI of gated pulmonary B) neutrophils and C) macrophages from both stimulated and unstimulated samples was measured by flow cytometry. Unstimulated values are shown, as well as the stimulation index (DHR MFI stimulated / DHR MFI unstimulated). Data shown are +/−SEM. *p<0.05, **p<0.01, ***p<0.001 and representative of three repeated experiments. Numbers of mice in representative figures; A-C) sham, n=4; burn, n=6.