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. 2018 Sep 25;7:e37382. doi: 10.7554/eLife.37382

Figure 1. Construction of inducible LHX6 OE hPSCs.

Figure 1.

(a) Schematic representation of electroporation to establish inducible LHX6 overexpressing (OE) hPSCs. (b) Bright-field images of hPSC colonies before and after electroporation. (c) After doxycycline induction, two inducible LHX6 OE hPSC cell lines expressed LHX6. Scale bar, 50 μm. (d) Schematic showing the differentiation of transgenic hPSC lines into dorsal neurons without adding morphogens. CON: default control group (−dox), LHX6 OE: LHX6 OE group (+dox). (e) mRNA expression levels for two transgenic hPSC-derived neurospheres and each control at day 17; n ≥ 3 for each cell line. (f–h) Representative images and quantification of transcription factors FOXG1 (f), PAX6 (g) and COUPTFII (h) expressed in CON and LHX6 OE neural precursors from two cell lines.