Figure 3. Ded1 acceleration of 48S PIC assembly is completely dependent on eIF4E·eIF4G for a subset of mRNAs.

(A–H) Apparent rates (k_app) of 48S PIC assembly for the indicated mRNAs observed without eIF4E·eIF4G and Ded1 (tan bars), with Ded1 but no eIF4E·eIF4G (green bars), with eIF4E·eIF4G but no Ded1 (blue bars), or with both Ded1 and eIF4E·eIF4G (orange bars). Note the different Y-axis scale for -SL (F). See Figure 3—figure supplement 1 and Figure 3—source data 1.

Figure 3—figure supplement 1. eIF4A is required for appreciable recruitment of all mRNAs tested (except -SL) in the presence or absence of saturating Ded1.

Recruitment of mRNAs without eIF4A in the absence (red) or presence of Ded1 (blue), and with eIF4A in the absence (green) or presence of Ded1 (cyan). The data were fitted with a single exponential equation for the reactions including eIF4A in the absence (green) and presence of Ded1 (cyan); and for all four conditions for -SL mRNA (H). Each point represents the mean value (n ≥ 2) and error bars are 1 SD from the mean. Note the x-axis is discontinuous. See Figure 3—figure supplement 1—source data 1.