Figure 5. RNA2 domain of eIF4G is crucial for Ded1-dependent stimulation of mRNA recruitment.

(A) k_max in the absence (blue) and presence of saturating Ded1 (orange) with eIF4E·eIF4G-${\displaystyle \mathrm{\Delta }}$RNA2. (B) The average fold-change in the maximal rate observed in the presence and absence of Ded1 with WT eIF4E·eIF4G (dark grey) and eIF4E·eIF4G-${\displaystyle \mathrm{\Delta }}$RNA2 (light grey). Red line indicates no change in the rate on Ded1 addition. (C) The K_1/2 of eIF4E·eIF4G-${\displaystyle \mathrm{\Delta }}$RNA2 in the absence of Ded1 (dark blue) and presence of saturating Ded1 (red). (A–C) Bars represent mean values (n = 3) and points on each bar show the individual experimental values. The line and whisker plot indicate the mean maximal rates (A) or K_1/2 (C) observed with WT eIF4E·eIF4G, and error bars represent 1 SD, as depicted in Figure 4. See Figure 4—figure supplements 1 and 2 and Figure 5—source data 1.