Figure 6. Ded1-NTD enhances mRNA recruitment.

(A) k_max values observed in the absence (blue) and presence of saturating Ded1-${\displaystyle \mathrm{\Delta }}$NTD (purple). (B) The average fold change in the maximal rate ${\displaystyle ({\mathrm{k}}_{\mathrm{m}\mathrm{a}\mathrm{x}}^{+\mathrm{D}\mathrm{e}\mathrm{d}1}/{\mathrm{k}}_{\mathrm{m}\mathrm{a}\mathrm{x}}^{-\mathrm{D}\mathrm{e}\mathrm{d}1})}$ observed in the presence and absence of WT Ded1 (dark grey) and Ded1- ${\displaystyle \mathrm{\Delta }}$NTD (light grey). Red line indicates no change in the rate with Ded1. (C) K_1/2 of Ded1-${\displaystyle \mathrm{\Delta }}$NTD value shown as green bars. (A–C) Bars represent the mean values (n = 3) and points on each bar show the individual experimental values. The line and whisker plot indicate the mean maximal rates (A) or K_1/2 (C) observed with WT Ded1, and error bars represent 1 SD. See Figure 4—figure supplements 1 and 2 and Figure 6—source data 1.