Figure 10.

L. plantarum attenuates CEF-induced cytokine, chemokine, and TLR-4 gene expression in liver by EGFR-dependent mechanism. A–H) Adult rtTA and rtTA-EGFR*-Tg mice were fed liquid diet with EtOH (EF) or isocaloric maltodextrin (PF), with or without L. plantarum (10⁶ CFU/ml) (LP) and with or without doxycycline (Dox). RNA extracted from liver was subjected to qPCR for TNF-α (A), IL-1β (B), IL-6 (C), IL-10 (D), MCP1 (E), CXCL1 (F), CXCL2 (G), and CCL5 (H) genes. I, J) TLR4 mRNA (M) was evaluated in liver of WT mice (I) or rtTA mice with or without EGFR* expression (J). Values are means ± sem (n = 4 for PF and EF and 6 for LP + EF and Dox + LP + EF groups). *P < 0.05, significantly different from corresponding PF values; ^#value different from corresponding EF values; ^Δvalue different from corresponding values for EF + LP without Dox.