Figure 2.

L. plantarum attenuates CEF-induced protein thiol oxidation and suppression of antioxidant gene expression in mouse colon. Adult female mice were fed liquid diet with EtOH (EF) or isocaloric maltodextrin (PF) and with or without L. plantarum (10⁶ CFU/ml) (LP). Cryosections of distal colon were stained for reduced and oxidized protein thiols (A) and fluorescence densities quantitated (B). RNA extracted from colonic mucosa was subjected to qPCR for SOD1 (C), SOD2 (D), Catalase (E), Gpx1 (F), Gpx3 (G), and Nrf2 (H) genes. Values are means ± sem (n = 6). *P < 0.05, significantly different from corresponding PF values; ^#value different from corresponding EF values.