Figure 7.

Expression of dominant negative EGFR attenuates L. plantarum–mediated prevention of CEF-induced expression of inflammatory cytokine and chemokine genes in mouse colon. Adult rtTA and rtTA-EGFR*-Tg mice were fed liquid diet with EtOH (EF) or isocaloric maltodextrin (PF), with or without L. plantarum (10⁶ CFU/ml) (LP) and with or without doxycycline (Dox). RNA extracted from colonic mucosa was subjected to qPCR for TNF-α (A), IL-1β (B), IL-6 (C), MCP1 (D), CCL5 (E), and CXCL2 (F) genes. Values are means ± sem (n = 4 for PF and EF and 6 for LP + EF and Dox + LP + EF groups). *P < 0.05, significatnly different from corresponding PF values; ^#value different from corresponding EF values; ^Δvalue different from corresponding values for EF + LP without Dox.