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. 2018 May 18;32(11):5793–5812. doi: 10.1096/fj.201700818RRR

Figure 9.

Figure 9

PGE2 elevates intracellular cAMP in ECs and induces permeability of dermal microvessels. Mice were injected with PGE2 (20 or 200 ng) or vehicle (DMSO) into dorsal skin on d 0 and d 1, then received intravenous injection of FITC-dextran (1 mg/ml) on d 2 to examine vessel permeability. Skin samples were collected 30 min after FITC-dextran injection, and tissue sections were immunostained for CD31 (red) and cAMP (magenta). Two representative micrographs of microvessels are shown for each condition and staining. Plasma leakage was assessed by quantitative fluorescence analysis of FITC-dextran outside of vessels. Degree of dextran leakage is presented in graph using arbitrary units. Analysis was conducted in >40 micrographs of different areas in 4 skin samples for each condition. Scale bar, 20 µm. Ns, not significant. *P < 0.05, **P < 0.01 (1-way ANOVA).