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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Neurochem. 2018 Sep 26;147(1):84–98. doi: 10.1111/jnc.14563

Fig 3. Effect of 1 week of withdrawal from repeated morphine exposure on the RhoA network in the NAc.

Fig 3

(a) Experimental design. Mice were intraperitoneally injected with saline or morphine 1x per day for 7 consecutive days. 1 week following the final injection P1 fractions were derived from NAc lysates.

(b and c) Western blots and quantification indicate that 1 week of morphine withdrawal does not affect PDZ-RhoGEF (t=0.3266, p>0.05), RhoA (t=0.1282, p>0.05), or Rock1 (t=0.02593, p>0.05) levels in NAc P1 fractions relative to saline-treated mice. n=12 saline mice, 11 morphine mice.

(d and e) Western blots and quantification indicate that 1 week of morphine withdrawal did not alter the expression of MAL (t=0.1606, p>0.05) or SRF (t=0.2064, p>0.05) in NAc P1 fractions. n=12 saline mice, 11 morphine mice

(f) Experimental design. Mice were intraperitoneally injected with saline or morphine 1x per day for 7 consecutive days. 1 week following the final injection S2 fractions were derived from NAc lysates.

(g and h) Western blots and quantification indicate that 1 week of morphine withdrawal did not affect the levels of PDZ-RhoGEF (t=1.991, p>0.05), RhoA (t=0.8842, p>0.05), or Rock1 (t=0.4595, p>0.05) in NAc S2 fractions. n=12 saline mice, 11 morphine mice.

(i and j) Western blots and quantification indicate that 1 week of morphine withdrawal did not alter the expression of MAL (t=0.4299, p>0.05) or SRF (t=1.918, p>0.05) in NAc S2 fractions. n=12 saline mice, 11 morphine mice.

(k) Experimental design. Mice were intraperitoneally injected with saline or morphine 1x per day for 7 consecutive days. 1 week following the final injection P2 fractions were derived from NAc lysates.

(l and m) Western blots and quantification indicate that 1 week of morphine withdrawal did not affect the levels of PDZ-RhoGEF (t=0.4244, p>0.05), RhoA (t=0.3352, p>0.05), or Rock1 (t=0.07281, p>0.05) in NAc P2 fractions. n=12 saline mice, 11 morphine mice.

All summary data are the mean + SEM.