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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Neurochem. 2018 Sep 26;147(1):84–98. doi: 10.1111/jnc.14563

Fig 5. Effect of the RhoA network on dendritic spines in NAc MSNs.

Fig 5

(a) Experimental design. HSV-GFP and HSV-RhoA-GEF-GFP were infused into the NAc. 5 days post viral infusion, NAc MSNs were imaged.

(b) Representative image shows dendritic segment from a NAc MSN infected with HSV-GFP. Different spine subtypes, including thin, stubby, and mushroom spines, are indicated.

(c) Representative images of NAc MSN dendritic segments from mice infused with HSV-GFP or HSV-RhoA-GEF-GFP into the NAc.

(d) Quantification indicates that NAc MSNs expressing HSV-RhoA-GEF-GFP show a significant reduction in the density of thin spines relative to HSV-GFP controls. The density of stubby and mushroom spines was not affected by this manipulation (two-way ANOVA, main effect of RhoA-GEF-GFP overexpression F=6.633, *p<0.05; Bonferroni posthoc thin spines, *p<0.05, Bonferroni posthoc stubby and mushroom spines, p>0.05). n=34 GFP cells and 15 RhoA GEF cells.

All summary data are the mean + SEM.