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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: Mol Microbiol. 2018 Sep 15;109(5):633–641. doi: 10.1111/mmi.14002

Figure 1. WzxC variants can substitute for MurJ.

Figure 1

Cells of CS7 [Para::murJ] harboring plasmids encoding C-terminally FLAG-tagged WzxC (WT) or the indicated derivatives were grown in LB medium with arabinose overnight. Following normalization for culture density, serial dilutions (10−1 to 10−6) were prepared and 5 μl of each were spotted onto LB plates supplemented with either glucose or IPTG. Plates were photographed after incubation at 37°C for ≈16 hours. All of the strains grew similarly on plates supplemented with arabinose under this condition. An additional growth experiment with additional concentrations of IPTG are presented in SI Appendix, Fig. S2.