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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: Mol Microbiol. 2018 Sep 15;109(5):633–641. doi: 10.1111/mmi.14002

Figure 2. Growth and morphology of ΔmurJ cells producing WzxC variants.

Figure 2

A. Cells of TB28 [WT], CS3/pDF2 [ΔmurJ/Plac::wzxC(V252M)-flag], and CS3/pDF3[ΔmurJ/Plac::wzxC(A33V)-flag] were grown in LB supplemented with 1 mM IPTG at 30°C in a 96-well plate. Growth was monitored by regular OD600 measurements in a plate reader. Plots are of the means of 10 technical replicates, and the error bars show the standard deviation. B–D. Cells of the same strains grown to an OD600 of 0.2–0.3 were imaged by phase-contrast microscopy. Bar equals 4 μm.