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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Clin Immunol. 2018 Apr 11;196:97–102. doi: 10.1016/j.clim.2018.04.003

Figure 1. Low transmethlyation micronutrient levels increase CD70, KIR and perforin expression by CD4+ and CD8+ T cells from healthy subjects.

Figure 1

(A) PBMCs were isolated from 9 healthy donors, stimulated with PHA and cultured in either standard RPMI 1640 or custom media with low transmethylation micronutrient levels as described in “Materials and Methods”. The cells were then stained with fluorochrome conjugated antibodies to CD4 or CD8 and analyzed by flow cytometry. (B) PBMC from 5 healthy subjects were similarly stimulated with PHA, cultured in either complete RPMI 1640 or the custom media then separated into CD4+ and CD8+ subsets as in panel A. Total RNA was then extracted and analyzed by real-time RT-PCR. Results are shown relative to expression by T cells cultured in the complete RPMI 1640. Error bars represent SEM.