Table 10.
Primer sequences used for real-time PCR.
| Gene | Forward (5′-3′) | Reverse (5′-3′) | Product length (bp) | Accession numbers |
|---|---|---|---|---|
| TLR4 | TCAGTTCTCACCTTCCTCCTG | GTTCATTCCTCACCCAGTCTTC | 166 | GQ503242.1 |
| MD2 | TGCAATTCCTCTGATGCAAG | CCACCATATTCTCGGCAAAT | 227 | NM_001104956.1 |
| CD14 | CGTTTGTGGAGCCTGGAAG | TGCGGATGCGTGAAGTTG | 226 | NM_001097445.2 |
| LBP | GAACACAGCCGAATGGTCTAC | GGAAGGAGTTGGTGGTCAGT | 151 | NM 001128435.1 |
| MyD88 | GATGGTAGCGGTTGTCTCTGAT | GATGCTGGGGAACTCTTTCTTC | 148 | AB292176.1 |
| IRAK1 | CAAGGCAGGTCAGGTTTCGT | TTCGTGGGGCGTGTAGTGT | 115 | XM_003135490.1 |
| TRAF6 | CAAGAGAATACCCAGTCGCACA | ATCCGAGACAAAGGGGAAGAA | 122 | NM_001105286.1 |
| RP105 | CGAGGCTTCTGACTGTTGTG | GGTGCTGATTGCTGGTGTC | 245 | AB190767.1 |
| SOCS1 | GCGTGTAGGATGGTAGCA | GAGGAGGAGGAGGAGGAAT | 101 | NM_001204768.1 |
| Tollip | GCAGCAGCAACAGCAGAT | GGTCACGCCGTAGTTCTTC | 133 | AB490123.1 |
| SIGIRR | ACCTTCACCTGCTCCATCCA | TTCCGTCATTCATCTCCACCTC | 205 | AB490122.1 |
| NOD1 | CTGTCGTCAACACCGATCCA | CCAGTTGGTGACGCAGCTT | 57 | AB187219.1 |
| NOD2 | GAGCGCATCCTCTTAACTTTCG | ACGCTCGTGATCCGTGAAC | 66 | AB195466.1 |
| RIPK2 | CAGTGTCCAGTAAATCGCAGTTG | CAGGCTTCCGTCATCTGGTT | 206 | XM_003355027.1 |
| NF-κB | AGTACCCTGAGGCTATAACTCGC | TCCGCAATGGAGGAGAAGTC | 133 | EU399817.1 |
| ERBB2IP | ACAATTCAGCGACAGAGTAGTG | TGACATCATTGGAGGAGTTCTTC | 147 | GU990777.1 |
| ACAP1 | GAAGCCGAAGTGTCCGAATT | AGGTCACAGATGCCAAGAATG | 125 | XM_003358258.2 |
| GAPDH | CGTCCCTGAGACACGATGGT | GCCTTGACTGTGCCGTGGAAT | 194 | AF017079.1 |