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. 2018 Oct 11;9:4206. doi: 10.1038/s41467-018-06185-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The presence or absence of a single cysteine residue defines the capacity of collagen-I C-Pro domains to form stable, disulfide-linked homotrimers. a Immunoblot analysis of individually expressed wild-type, HA-tagged C-Proα1(I) (red) and wild-type, FLAG-tagged C-Proα2(I) (green) proteins under non-reducing and reducing conditions showing that wild-type C-Proα1(I) forms a disulfide-linked homotrimer, whereas wild-type C-Proα2(I) does not, recapitulating the known disulfide-dependent assembly patterns of full-length Colα1(I) and Colα2(I). Co-expression of wild-type C-Proα1(I) and wild-type C-Proα2(I) rescues wild-type C-Proα2(I) into a heterotrimer, as shown by the yellow color indicating red and green overlap. b Immunoblot analysis of individually expressed HA-tagged Cys1265Ser (C2S) C-Proα1(I) (red) and FLAG-tagged Ser1169Cys (S2C) C-Proα2(I) (green) proteins under non-reducing and reducing conditions showing that the serine variant of C-Proα1(I) is no longer able to form a disulfide-linked homotrimer. In contrast, the cysteine variant of C-Proα2(I) is able to form disulfide-linked homotrimers. Co-expression of C2S C-Proα1(I) and S2C C-Proα2(I) rescues C2S C-Proα1(I) into a heterotrimer, as shown by the yellow color indicating red and green overlap