Table 2.

Steady-state kinetic parameters for one-base incorporation catalyzed by human wild-type and mutant polκ.

Base pairing	Polκ1–526	K m (μM)	k cat (min−1)	kcat/Km (min−1 μM−1)	Efficiency relative to WT
T:dATP	WT	14.9 ± 1.2	38.1 ± 1.4	2.56	1
T:dATP	K25A	33.1 ± 2.8	4.2 ± 0.2	0.13	0.05
T:dATP	R149A	57.0 ± 4.4	18.3 ± 1.9	0.32	0.13
BP-dG:dCTP	WT	1247 ± 121	4.1 ± 0.1	3.3 × 10−3	1
BP-dG:dCTP	K25A	1328 ± 97	0.050 ± 0.003	3.8 × 10−5	0.01