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. 2018 Oct 11;8:15157. doi: 10.1038/s41598-018-33566-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of OCLN expression on tight junction permeability. (A and B) A549 and A549/CDDP cells were plated on transwell inserts. As indicated, A549/CDDP cells were treated with or without of 10 μM LY-294002 (LY) for 24 h. TER and paracellular DXR flux were analyzed by a volt ohmmeter and fluorescence spectrometry, respectively. (C) Cell lysates were prepared from empty (mock) or FLAG-tagged OCLN-expressing cells. The expression of OCLN, FLAG, and β-actin was examined by western blotting. The protein levels of OCLN are shown as a percentage of the values in mock cells. The full-length blot images are shown in Supplementary Fig. S3. (D) TER and the paracellular DXR flux were measured in mock and OCLN-expressing cells. n = 3–4. **P < 0.01 and *P < 0.05 compared with A549. NS, P > 0.05.