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. 2018 Oct 11;8:15156. doi: 10.1038/s41598-018-33509-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Developmental change in the cell type-specificity and reporter expression efficacy of the three different lentiviral promoters. (a) The ratio of TagRFP-positive populations within GABAergic neurons obtained using the synapsin I, α-CaMKII and 2.1 kb VGLUT1 promoters at three different stages in culture. There were no significant changes in any of the groups that were tested. (b) The percentage of TagRFP-expressing neurons in the glutamatergic (i.e., Venus-negative) population obtained using the synapsin I, α-CaMKII and 2.1 kb VGLUT1 promoters at three different stages in culture. The reporter expression efficacy of the VGLUT1 promoter significantly increased by the middle of the third week in vitro (*p < 0.05, One-way ANOVA followed by Bonferroni’s test).