Figure 4.

SF1-STE pairing mediates enhancer bypass. (A) Diagram of the enhancer-bypass transgene containing SF1and STE placed in tandem between NEE and H1 in the pWNHZ vector (red ovals, also see Methods). (B–G) Representative images of transgenic embryos after whole mount in situ hybridization with anti-lacZ probes. DNA elements in these embryos are labeled at bottom right of each photo and probes used on bottom left. Orientation of the elements relative to the endogenous arrangement is indicated by “f” (forward) or “r” (reverse). (H) Quantitation of NEE-blocking in by tandem insulators in bypass transgenic embryos (see Methods for details).