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. 2018 Oct 11;8:15177. doi: 10.1038/s41598-018-33487-8

Figure 2.

Figure 2

Replication of ORF8 variants in primate cell cultures with and without IFN pre-treatment. (a) VeroFM and MA104 cells were infected with three ORF8 variant viruses at MOI = 0.001. Supernatants were harvested at 24 hpi and virus titers determined by plaque titration. The experiment was done in triplicates. Significance of replication differences between virus variants was determined by t-Test (***p < 0.001, *p < 0.05). (b) VeroFM cells were incubated with universal type I IFN at indicated concentrations 16 h prior to infection with rSCV8full and rSCVdel8 at MOI = 0.001. At 24 hpi supernatants were plaque titered. Experiments were done at least twice in triplicates. Shown is one representative experiment each. Error bars represent standard deviation of the mean.