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. 2018 Oct 11;7(10):82. doi: 10.1038/s41389-018-0092-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Dot blot analysis of CpG methylation levels in HCT116 (black bar), U2OS (grey bar), and HeLa (light grey bar) cells treated with siRNA against ZBTB38 or control siRNA and further exposed to 5-azacytidine (n = 3). The data represent relative levels of the condition siRNA ZBTB38 versus siRNA control. b Dot blot analysis of CpG hydroxymethylation levels in HCT116 (black bar), U2OS (grey bar), and HeLa (light grey bar) cells treated with siRNA against ZBTB38 or control siRNA and further exposed to 5-azacytidine (n = 3). The data represent relative levels of the condition siRNA ZBTB38 versus siRNA control. c Dot blot analysis of CpG methylation and hydroxymethylation levels in THP-1 cells treated with siRNA against ZBTB38 or control siRNA and further exposed to decitabine (n = 2). The data represent relative levels of the condition siRNA ZBTB38 versus siRNA control. d RT-PCR analysis of genes modulating 5-azacytidine toxicity in HCT116, U2OS, and THP-1 cells treated with siRNA against ZBTB38 (dashed bars) and control siRNA (solid bars) (n = 3)