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. 2018 Oct 11;7(10):82. doi: 10.1038/s41389-018-0092-0

Fig. 3. Inactivation of ZBTB38 by RNA interference enhances the cytotoxicity of 5-azacytidine in a variety of cancer cells.

Fig. 3

a Western blot analysis of ZBTB38 and GAPDH expression in cells treated with three different anti-sense RNA directed against ZBTB38 and a control anti-sense RNA. Relative expression level of ZBTB38 is indicated under the blots with the control condition set up as 1. b Depletion of ZBTB38 enhances the cytotoxicity of 5-azacytidine in colony formation assays of HeLa cells (n = 3). Colony were counted 15 days post-azacytidine exposure and normalized to the condition without 5-azacytidine, artificially set up as 1. ***P < 0.001; *P < 0.05. c Depletion of ZBTB38 enhances the cytotoxicity of 5-azacytidine in colony forming assays of HCT116, U2OS, DU145, HCT116 p53-−/− and HCT116 DKO cells (n = 4). ***P < 0.005. d Depletion of ZBTB38 enhances the cytotoxicity of 5-azacytidine in leukemia K562, THP-1, and MOLM-14 cells (n = 3). Cell viability was analyzed 2 days post-azacytidine exposure by scoring trypan blue-positive cells and reported as the number of viable cells normalized to the condition without 5-azacytidine, artificially set up as 1. ***P < 0.001; *P < 0.05