Fig. 4. Inactivation of ZBTB38 by RNA interference causes a strong arrest in cell proliferation upon DNMTi exposure, persistant after removal of DNMTi.

a Graph depicting the growth curve of K562 cells treated with siRNAs (control and ZBTB38) and with decitabine 1 µM (DAC) or not (n = 4). ***P < 0.001. b, c Same experiment in THP-1 (b) and MOLM-14 (c). d Graph depicting the proliferative potential of THP-1, MOLM-14, and K562 cells transfected with siRNAs (control and ZBTB38) and further exposed to decitabine (DAC) or azacytine (AZA) (n = 4). ***P < 0.001. *P < 0.05. e Graph depicting the proliferative potential of THP-1 (left panel) and MOLM-14 (right panel) cells transfected with siRNAs (control and USP9X) and further exposed to decitabine 1 µM (DAC) (n = 4). ***P < 0.001