Fig. 5. SOCS6 was a target gene of miR-21-5p in BC cells.

a The predicted miR-21-5p binding site in the 3′UTR of SOCS6 mRNA. Short vertical lines indicated complementary nucleotides. b The relative luciferase reporter assay of HEK 293 T cells co-transfected with pmiR-SOCS6-W or pmiR-SOCS6-M and agomiR-21-5p or agomiR-NC. **P < 0.01 vs. pmiR-SOCS6-W + agomiR-NC group. c The expression of SOCS6 protein in KK47 and T24 cells. *P < 0.05 vs. agomiR-NC group, **P < 0.01 vs. agomiR-NC group; ^#P < 0.05 vs. antagomiR-NC group, ^##P < 0.01 vs. antagomiR-NC group. d The expression of SOCS6 protein in KK47 and T24 cells. **P < 0.01 vs. pUC-NC + agomiR-NC group; ^##P < 0.01 vs. pUC-NBAT1 + agomiR-NC group