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. 2018 Oct 11;8:164. doi: 10.1186/s13568-018-0694-0

Table 2.

Optimal concentration combinations of four trace elements affecting the proliferation of Paenibacillus polymyxa Pp-7250

Test number Cu2+ (A) Fe2+ (B) Zn2+ (C) Mn2+ (D) OD600 60 h
Results of the orthogonal experiment
 1 1 1 1 1 0.9379
 2 1 2 2 2 1.0552
 3 1 3 3 3 0.7374
 4 2 1 2 3 0.6545
 5 2 2 3 1 0.6614
 6 2 3 1 2 1.2293
 7 3 1 3 2 0.7192
 8 3 2 1 3 0.8526
 9 3 3 2 1 0.9106
 T1 2.7305 2.3116 3.0198 2.5099 7.7581
 T2 2.5452 2.5692 2.6203 3.0037
 T3 2.4824 2.8773 2.1180 2.2445
 The average k1 0.9102 0.7705 1.0066 0.8366
 k2 0.8484 0.8564 0.8734 1.0012
 k3 0.8275 0.9591 0.7060 0.7482
 R value 0.0827 0.1886 0.3006 0.2530
SS df MS F F0.05(2,2) Significant
Variance analysis
 A 0.0111 2 0.0056 56.0000 19.0000 *
 B 0.0534 2 0.0267 267.0000 **
 C 0.1361 2 0.0681 681.0000 ****
 D 0.0989 2 0.0495 495.0000 ***
 Error 0.0001 2