Figure 2.

The expression of NLRC5, RIG-I, and MDA5 is inducible in primary human pDCs after CpG-A treatments. (A–D) Freshly isolated primary human pDCs were stimulated with 2.5 μM CpG-A for 16 h, thereafter the protein levels of NLRC5, NLRX1, RIG-I, MDA5, MAVS, and TBK1 were detected by western blotting. Representative blots are shown in (A,C). Data are shown as mean ± SD from 3 to 4 experiments in panels (B,D). (B,D) Statistical comparisons were performed using Student's t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.