Skip to main content
. 2018 Oct 5;9:2200. doi: 10.3389/fimmu.2018.02200

Figure 1.

Figure 1

Poly (I:C)-elicited inflammation enhances the magnitude and rate of alloimmunization to HOD RBCs. Recipient B6 mice were treated i.p. with 200 ug of poly (I:C) or PBS control 2–4 h prior to a transfusion of 100 uL packed leukoreduced HOD RBCs. Sera was collected and assessed for anti-HOD alloantibodies by flow crossmatch against HOD RBC targets. The adjusted mean fluorescence intensity (MFI) of anti-mouse Igs APC secondary staining reagent was determined for days (A) 7 and (B) 14 post RBC transfusion. (C) Reactivity of day 14 serum to HOD RBC targets from individual mice in each group is shown by flow crossmatch against HOD RBC targets with total anti-mouse Igs and (D) subtype specific IgG secondary reagents. Naïve B6 sera incubated with HOD RBC targets is shown in shaded gray, poly (I:C) treated mice in red, and PBS treated animals in blue; each line represents an individual mouse. This experiment was repeated 3 times with 3–5 mice per group and all experiments are shown. Sera was used at 1:100 dilution. *p ≤ 0.05.