Figure 2.

TAMRA-pPy-stained λ DNA tethered on the surface of a flow cell. (A) Free-floating λ DNA (48.5 kb) molecules with a mushroom-like conformation in the flow off condition. (B) Fully elongated λ DNA molecules with a flow of 100 μl/min. Arrows indicate flow direction. Scale bar 10 μm. (C) Comparison of experimentally measured fluorescence intensity (red) with in silico sequence frequencies from the λ genome sequence. The black solid line represents A/T (W), the gray dotted line indicates four consecutive A/T (W₄), and the grey solid line is W₉. (D) Cross-correlation (cc) coefficient values calculated from the alignment of 20 molecular images with the genome using three kinds of binding sequences (W, W₄ and W₉). The control cc was obtained from 100 computer-generated random sequences (***P < 0.0001 for random-sequences paired t-test).