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. 2018 Jul 16;46(18):9736–9748. doi: 10.1093/nar/gky636

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Conformational dynamics of ribosomal complexes on non-slippery mRNAs with different pseudoknots in the first pseudoknot-unwinding cycle. (A) Dwell time distributions of the non-ratcheted and ratcheted POST^F states. (B) Dwell time distributions of the non-ratcheted and ratcheted PRE^FV states. (C–F) Plots of dwell times of non-ratcheted POST^F (C), ratcheted POST^F (D), non-ratcheted PRE^FV (E) and ratcheted PRE^FV (F) captured by smFRET under equilibrium conditions versus frameshifting efficiency. n is the number of events. Dwell times were extracted by single exponential fitting of dwell time distributions except the non-ratcheted POST^F, whose dwell times were extracted using double exponential decay. Error bars were standard errors.