Table 1.
-1 PRF efficiency and dwell time of ribosomal complexes during ongoing elongation experiments
| Dwell time in the first cycle | Dwell time in the second cycle | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| –1 PRF efficiency (%)a | non-slippery sequence | slippery sequence | non-slippery sequence | ||||||
| mRNA structure | Single-molecule counting | Ensemble assay | PREFV (s) | POSTFV (s) | PREFV + POSTFV (s) | PREK1K2 (s) | POSTK1K2 (s) | PREVR (s) | POSTVR (s) |
| PL | 8 ± 1 | 10 ± 3 | 1.1 ± 0.1 | 2.9 ± 0.4 | 2.6 ± 0.4 | 1.6 ± 0.3 | 1.5 ± 0.3 | 1.3 ± 0.1 | 2.5 ± 0.3 |
| PT2G32 | 16 ± 2 | 19 ± 5 | 0.9 ± 0.2 | 4.6 ± 0.5 | 5.0 ± 0.5 | 2.3 ± 0.1 | 2.1 ± 0.2 | 1.4 ± 0.1 | 3.2 ± 0.4 |
| PEMV1 | 29 ± 3 | 23 ± 5 | 0.9 ± 0.1 | 5.7 ± 0.5 | 7.7 ± 0.6 | 2.3 ± 0.2 | 2.9 ± 0.2 | 1.5 ± 0.1 | 4.1 ± 0.4 |
| ScYLV | 61 ± 3 | 63 ± 7 | 0.7 ± 0.1 | 7.8 ± 0.3 | 9.6 ± 0.7 | 2.2 ± 0.3 | 4.0 ± 0.2 | 1.6 ± 0.1 | 3.8 ± 0.5 |
| HERV | 45 ± 2 | 48 ± 8 | 0.8 ± 0.1 | 8.6 ± 0.3 | 9.4 ± 0.8 | 2.0 ± 0.1 | 3.6 ± 0.1 | 1.5 ± 0.1 | 4.2 ± 0.6 |
| VMV | 44 ± 3 | 39 ± 3 | 0.9 ± 0.1 | 7.7 ± 0.8 | 9.1 ± 0.3 | 2.3 ± 0.3 | 3.2 ± 0.4 | 1.5 ± 0.1 | 3.3 ± 0.4 |
| Pearson correlation coefficientb | 0.73 (0.10) | 0.92* (0.01) | 0.93* (0.01) | 0.43 (0.39) | 0.97* (0.01) | 0.94* (0.01) | 0.66 (0.15) | ||
Mean ± SEM was calculated from three to four independent replicates.
a–1 PRF efficiency was measured in the presence of the slippery sequence (A AAA AAG).
bPearson correlation coefficient between dwell times of each ribosomal complex and -1 PRF efficiency measured by single-molecule counting. Corresponding P-value was listed within parentheses. * indicated significant correlation.