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. 2018 Jun 19;46(18):e111. doi: 10.1093/nar/gky558

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — SES-based production of CBHI protein in Trichoderma reesei. (A) A schematic presentation of the SES-C cassette, including position of the HPH selection marker and cbh1-locus genome-integration flanks, used for production of CBHI enzyme in T. reesei. (B) The analysis of total protein produced (secreted) in bioreactor cultivations of T. reesei. The parental (PS) as well as the SES strain were cultivated in cellulase-inducing medium (containing lactose and spent grain, SGM), and repressing medium (containing glucose). Culture media samples were analysed for total produced protein by SDS-PAGE gel and Coomassie staining (left panel). The total protein concentrations were calculated based on the purified CBHI protein standard (right panel). (C) The analysis of the CBHI protein produced (secreted) in bioreactor cultivations of T. reesei. The samples of B) were analysed by a western blot (left panel) with a specific anti-CBHI (mab261) antibody. The CBHI concentrations were calculated based on the purified CBHI protein standard (right panel).