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Recruitment dynamics of SPOP and key effectors in DNA repair in response to laser-induced double-strand breaks. (A) U2OS cells expressing doxycycline-inducible FLAG-SPOP were microlaser irradiated, fixed at the indicated time points and stained by immunofluorescence for γH2AX and FLAG. U2OS cells were transfected with siRNA and microlaser irradiated, fixed after 1 h and stained by immunofluorescence for γH2AX, RPA70 (B) and RAD51 (C). Scale bars, 10 μm.
