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. 2018 Aug 8;46(18):9805–9815. doi: 10.1093/nar/gky702

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — The Cas1–Cas2 complex and Csn2 comprise the type II-A CRISPR adaptation module. (A) The interaction between the Cas1–(His)₆-MBP-Cas2 complex (10 μM) and Csn2 (40 μM) was assessed by analytical SEC. Elution fractions were analyzed by SDS-PAGE. Uncropped gel images are shown in Supplementary Figure S18. (B) ITC trace for the binding of the Cas1–(His)₆-MBP-Cas2 complex to Csn2. Csn2 (50 μM) was titrated with the Cas1–(His)₆-MBP-Cas2 complex (250 μM). The experimentally determined N and K_d values are indicated.