Figure 5.

Co-transfected siRNAs compete with one another in a predictable manner. (A) A titration experiment was set up to establish a dose-response curve for a standard siRNA targeting the KIF11 transcript, which has a strong on-target effect (cell death) and is thus used as a transfection control in many screens. (A, B) The black curve from (A) was repeated with various oligos co-transfected at a constant amount, and the horizontal shift of the dose-response curve was measured. (C) Significance of shifts in concentration response curves upon co-transfecting various classes of siRNAs (in each pairing, the left oligo was titrated, and the right was given at a constant amount; FC = fold change, dividing the right oligo's inflection point by the left oligo's inflection point). (D) Correlation plot between shifts in concentration response curves of siRNAs targeting KIF11 upon co-transfection with customized siRNAs, and decrease in cell number upon transfection with these customised oligos alone.