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. 2018 Jul 13;46(18):9726–9735. doi: 10.1093/nar/gky628

Figure 6.

Figure 6.

Loss of inherited Cyrano by ectopic over-expression of miR-7 leads to defective brain development in 24 hpf embryos. (A) HuC: kaede zebrafish injected with control or miR-7 mimic imaged at 24 hpf to capture GFP labelled medial longitudinal fasciculus (MLF), nucleus of medial longitudinal fasciculus (nMLF) and trigeminal ganglion (tg). (B) Distance between MLF and (C) nMLF in control and miR-7 mimic over-expressed embryos measured, both the regions shown by red boxes in A. (D) Distance between left tg to nMLF and (E) right tg to nMLF in control and miR-7 mimic over-expressed embryos measured, as shown by red lines in A. Error bar represents standard error of mean, n = number of animals, **P value ≤ 0.01, *P value ≤ 0.05.