Figure 10.

si19-37 annealing stabilizes HCV genomic RNA in Ago2 knockout cells. Ago2 cells were elecroporated with non-replicative HCV RNA and the indicated siRNAs and miRNA antagonists, anti-122 or the control anti-124. RNA was prepared from the cells at the indicated short term time points post-electroporation and probed for HCV and Actin RNA to evaluate HCV RNA stability. A representative northern blot analysis of an HCV RNA stability assay is shown in (A) and the quantification of three independent assays is shown in (B).