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. 2018 Jul 24;46(18):9776–9792. doi: 10.1093/nar/gky662

Figure 8.

Figure 8.

Sequence and replication analysis of viruses whose replication was promoted by the siRNA used in the mutation selection method. Viruses selected by si18-36 or si19-37 were tested for promotion by their respective selection siRNA. (A) Viruses whose replication was promoted by si18-36 and si19-37 when miR-122 was absent. The indicated viral RNAs were co-transfected with either si18-36, si19-37 or siControl into miR-122-knockout Huh-7.5 cells and replication was assessed based on luciferase expression. (B) The 5′ UTR sequences of viruses whose replication was promoted by si18-36 or si19-37, and the specific annealing between si18-36 and C26U are shown. (C–E) Time course analysis in wild-type and miR-122-knockout cells of C26U with various small RNAs. C26U RNA was co-electroporated into (C and E) wild-type or (D) miR-122-knockout Huh-7.5 cells with the indicated miRNA, siRNA or miR-122 antagonist (anti122). (F) Time course analysis of WT HCV RNA in miR-122-knockout cells with the indicated small RNAs.