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. 2018 Jul 3;46(18):9660–9666. doi: 10.1093/nar/gky598

Figure 4.

Figure 4.

Proposed binding of Z and Z-mod and effect of mutations in Z-mod. (A) Diagrammatic representation of the binding of Z to WXY in the case of non-modified substrates (20,29). (B) Proposed binding of Z-mod to WXY. (C) Sequence of mutated variants (M1-6, substitutions in red) of Z-mod and the yield of WXY-mod after 1 h reaction between WXY and non-mutated or mutated variants of Z-mod obtained by polyacrylamide gel electrophoresis. The reported yield is the substrate to product conversion in percentage. Error bars represent ±1 standard deviation (triplicates).