Figure 5.

Formation of cooperative RNA network using modified substrates. Reactions were started by adding WXY fragments with different IGS-tag sequences (MN = AA, GU and UC, right panel) as used in previous studies (30,31). The reaction is either provided with native (Z, purple) or modified substrate (Z-mod, orange). Kinetic analysis was performed by analysing the formation of WXYZ at different time points using polyacrylamide gel electrophoresis. The reported yield is the substrate to product conversion in percentage. Error bars represent ±1 standard deviation (triplicates).