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. 2018 Aug 27;41(9):830–841. doi: 10.14348/molcells.2018.0181

Fig. 2. MiR-766-3p up-regulation inhibits HCC cell proliferation, colony formation in vitro and tumour growth in vivo.

Fig. 2

(A) miR-766-3p expression in HCC cell lines (SMMC7721, SK-HEP-1, Hep3B, PLC/PRF/5 and Huh7) and a normal human liver epithelial cell line (LO2) was measured by qRT-PCR. (B) SK-HEP-1 and PLC/PRF/5 cells were transfected with the miR-766-3p expression plasmid (miR-766-3p) or non-targeting miRNA precursor plasmid (NC). miR-766-3p expression in SK-HEP-1 and PLC/PRF/5 cells was detected by qRT-PCR. U6 expression was used as a control. (C) Cell proliferation was tested with CCK-8. MiR-766-3p overexpression significantly inhibited the proliferation of SK-HEP-1 and PLC/PRF/5 cells. (D) MiR-766-3p overexpression significantly reduced the colony formation of SK-HEP-1 and PLC/PRF/5 cells. (E) MiR-766-3p overexpression in SK-HEP-1 cells significantly repressed tumour growth in vivo. *P < 0.05; **P < 0.01.