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Cryo-ET reconstructions of the whole cells from KK148 and KK148 ΔflgT exhibiting dramatic differences in flagellar structures. (A, B) Tomographic slice of KK148 ΔflgT shown in different layers of the tomogram. (C, D) Images from panels A and B in a 3D segmentation to show the periplasmic flagella. (E) Representative tomogram slice of a KK148 whole cell shows multiple polar sheathed flagella. (F) 3D segmentation of the image from panel E.
