TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Reference or source |
|---|---|---|
| V. alginolyticus | ||
| VIO5 | VIK4 (Rifr Pof+ Laf−) | 42 |
| KK148 | VIO5 flhG (multi-Pof+) | 43 |
| TH7 | KK148 ΔflgT | 14 |
| NMB337 | KK148 ΔflgO | This study |
| E. coli | ||
| DH5α | Recipient for DNA manipulation | 46 |
| β3914 | Recipient for conjugational transfer of pSW7848 | 44 |
| Plasmids | ||
| pGEM-T Easy | Cloning vector, Ampr | Promega |
| pSW7848 | Suicide vector, (oriVR6Kγ oriTRP4 araC-PBAD-ccdB) Cmr | 45 |
| pBAD33 | Cmr PBAD | 47 |
| pTSK127 | pGEM-T Easy-ΔflgO | This study |
| pTSK127_2 | pSW7848-ΔflgO | This study |
| pTY57 | Cmr, PBAD with a multicloning site of pBAD24 | 48 |
| pTSK128 | pTY57-flgO::His6 | This study |
a
Rifr, rifampin resistant; Pof+, normal polar flagellar formation; Laf−, defective in lateral flagellar formation; multi-Pof+, multiple polar flagellar formation; Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; PBAD, arabinose promoter.