| Column no. | Description |
|---|---|
| 1 | An identifier for the amplicon (must be unique) |
| 2 | Amplicon sequence used in the design of the experiment |
| 3 (optional) | sgRNA sequence used for this amplicon, without the PAM sequence. If not available, enter ‘NA’ |
| 4 (optional) | Expected amplicon sequence in the case of HDR. If more than one, separate by commas and not spaces. If not available, enter ‘NA’ |
| 5 (optional) | Subsequence(s) of the amplicon corresponding to coding sequences. If more than one, separate by commas and not spaces. If not available, enter ‘NA’ |
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