Table 6.

Nomenclature inconsistencies by Ion Reporter^™

Gene	Expected variant	Ion Reporter™ variant
KIT	c.1672_1674dupAAG p.(Lys558dup)	c.1670_1671insGAA p.([Lys558dup)]
	c.1679_1681delTTG p.(Val560del)	c.1675_1677delGTT p.(Val559del)
	c.1735_1737 p.(Asp579del)	c.1733_1735delATG p.(Asp579del)
	c.1730_1738del p.(Pro577_Asp579del)	c.1728_1736del p.(Pro577_Asp579del)
EGFR	c.2303_2311dup p.(Ser768_Asp770dup)	c.2300_2301insCAGCGTGGA p.(Ala767_Ser768insSerValAsp)
BRAF	c.1798_1799delGTinsAA p.(Val600Lys)	c.1798_1799delGTinsAA p.(Val600Lys) plus c.1798G > A p.(Val600Met)

Five variants were identified by the IonReporter^™ analysis workflow with the incorrect nomenclature and checked by Alamut Visual v2.7.1 (Interactive Biosoftware). A large proportion of nomenclature errors were deletions, insertions and duplications