Fig. 4.

Functional activity of expanded ovarian TILs against autologous tumour cells. a IFNγ release after 24-h co-culture of TILs with autologous tumour. TILs were considered to show functional activity if significantly higher levels of IFNγ were produced by the co-culture compared to TILs alone. Mean and SD of three replicates are shown. *p < 0.05, **p < 0.01, ***p < 0.001, paired Student’s t test. b Effect of MHC class I and II blockade on TIL functional activity against autologous tumour. Expanded TILs were co-cultured against autologous tumour cells that have been pre-incubated with either an anti-MHC class I or anti-MHC class II antibody, and IFNγ release compared. Representative results from three donors are shown. Mean and SD of three replicates are shown. *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA with multiple comparisons