Table 1.
Human primer sequences used for semi-quantitative RT-PCR
| Gene Name | Primer sequence - 3’-5’ | Annealing temperature (°C) | Base pair size (bp) |
|---|---|---|---|
| Corneal Epithelial Stem Markers | |||
| ABCG2 | FP: 5’ AGTTCCATGGCACTGGCCATA 3’ RP: 5’ TCAGGTAGGCAATTGTGAAGG 3’ |
62 | 379 |
| Cytokeratin 3 | FP: 5’ GGCAGAGATCGAGGGTCTC 3’ RP: 5’GTCATCCTTCGCCTGCTGTAG 3’ |
64 | 145 |
| Connexin 43 | FP:5´ CCTTCTTGCTGATCCAGTGC 3´ RP 5´ ACCAAGGACACCACCAGCAT 3´ |
63 | 150 |
| Antimicrobial peptide – AMP | |||
| hBD-1 | FP: GCCTCCAAAGGAGCCAGCGT RP: CTTCTGGTCACTCCCAGCTCA |
54 | 287 |
| hBD-2 | FP: CAGCCATCAGCCATGAGG RP: TGGCTTTTTGCAGCATTTT |
55 | 204 |
| hBD-3 | FP: AGCCTAGCAGCTATGAGGATC RP: CTTCGGCAGCATTTTCGGCCA |
61 | 205 |