Figure 7.

Mutations in clusters I‐III of RNAP β subunit detected in different B. subtilis strains. The bar represents the amino acid sequence of the RNAP β subunit (1193 aa). The PCR‐amplified and sequenced region (716‐bp from rpoB) included the hot‐spot Clusters I‐III associated to Rif^r substitutions (Campbell et al., 2001). The position of the Rif^r substitutions corresponding to aa regions 452‐490, 514‐516 and 649‐687 from RpoB are shown in brackets. The Cluster I in which most of the Rif^r mutations were detected is shown in underlined blue bold letters. The WT amino acid sequence for each strain analyzed is shown in light‐gray bold letters and the amino acid change associated with the Rif^r phenotype is shown in black bold letters. The frequency of a particular mutation is shown below each amino acid change. aa (amino acid)